Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants

Acute and postexposure effects of ammonia toxicity on juvenile barramundi (Lates calcarifer [Bloch]).

Lethal and sublethal effects of ammonia toxicity to juvenile barramundi (Lates calcarifer) were investigated under laboratory conditions following the OECD guidelines for testing of chemicals. Acute toxicity was tested in a static nonrenewal system at pH 9.0 and temperature around 29 degrees C. The 24-, 48-, and 96-h LC(50) values for barramundi were 3.89, 3.67, and 3.31 mg total ammonia N L(-1...

Comparison of glycerol, lactamide, acetamide and dimethylsulfoxide as cryoprotectants of Japanese white rabbit spermatozoa.

The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility an...

Diseases of Asian seabass ( or barramundi ) , Lates calcarifer Bloch Susan GIBSON - KUEH

Defining essential fatty acid deficiency in juvenile barramundi Lates calcarifer

Barramundi (Lates calcarifer), a catadromous teleost of significant and growing commercial importance, are reported to have limited fatty acid bioconversion capability, typical of marine species, and therefore require preformed long-chain polyunsaturated fatty acids (LC-PUFA) as dietary essential fatty acid (EFA). In this study, the response of juvenile barramundi (47.0 g/fish initial weight) f...

A microsatellite linkage map of Barramundi, Lates calcarifer.

Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (approximately 700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage g...